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Laser Scanning Confocal Microscope (LSCM)

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Olympus-LEXT

Contact details

Mookhoek, Steven ir.
Aerospace Engineering (LR), Fundamentals of Advanced Materials (FAM)
Kluyverweg 1, 2629 HS Delft
Tel : +31 (0)15 27 84703
E-mail : s.d.mookhoek@tudelft.nl
Website : Open website

General Information

Instrument:

Laser Scanning Confocal Microscope (LSCM)

Location:

Kluyverweg 1

Key words:

characterisation, surface, confocal, microscopy

Main application:

Confocal laser scanning microscopy (CLSM or LSCM) is a technique for obtaining high-resolution optical images.The key feature of confocal microscopy is its ability to produce in-focus images of thick specimens with texture, a process known as optical sectioning. Images are acquired point-by-point and reconstructed with a computer, allowing three-dimensional reconstructions of topologically-complex objects.

References:

http://www.olympusconfocal.com/theory/confocalintro.html

Instrument specification

 

Olympus LEXT OLS3100

 

Optics

Objectives: 5x, 10x, 20x, 50x, 100x

Optical zoom: 1X - 6X

Total magnification: 120X - 14400X

Field of view: 2560X2560um - 21X21um

 

Laser

408 nm LD (class 2)

 

Maximal resolution

  • x-y ~ 150 nm
  • z ~ 15 nm

 

Accessoires

  • Automatic movable stage
  • Automatic imaging (timelaps)
  • Surface analysis software

 

Maximum height of specimen: 100 mm